Galactosyltransferases

O Keefe, E.T., Mordick, T., and Bell, J.E. (1980) Bovine galactosyltransferase Interaction with a-lactalbu-min and the role of a-lactalbumin in lactose synthase. Biochemistry 19, 4962-4966. 

Fig. 15.7 Glycosylation of an antibody produced in tobacco plants expressing a human 3(l,4)-galactosyltransferase. As illustrated for Guy sl3 in Fig. 15.4, when the monoclonal antibody Mgr48 is produced in wild type tobacco plants (left panel), its glycosylation is structurally different and more heterogeneous than that of its mammalian counterpart (lower panel). When this antibody is produced in tobacco plants expressing the human galactosyltransferase (right panel), 30% of its N-glycans show terminal N-acetyllactosamine sequences identical to those carried by this antibody when it is produced in hybridoma cells.

Similarly Thiem and Wiemann (92) conducted the galactosylation of the 2-acetamido-2-deoxy-D-glucose-L-asparagine derivative 183 and the chitobiose-L-asparagine derivative 187 to furnish the di- and trisaccharide derivatives 185 and 188, respectively, by using galactosyltransferase. 

Sprang, H., Kruithof, B., Leijendekker, R., Slot, J. W., van Meer, G., and van der Sluijs, P., 1998, UDP-galactose ceramide galactosyltransferase is a class 1 integral membrane protein of the endoplasmic reticulum. J Biot Chem 273 25880-25888. 

Volume 32 ot-Gal and Anti-Gal al3-Galactosyltransferase, a-Gal Epitopes, and the Natural Anti-Gal Antibody... 

Scheme 9.—Incorporation of o-Galactose into a /3-D-Glucopyranosyl Polymer Catalyzed by /3-D-Galactosyltransferase (EC 2.4.1.22), Followed by Photochemical Release of Lactose.

T r) seeds [10]. O gives brown, whereas recessive o gives a red-brown phenotype. Preliminary analysis reveals the anthocyanin content of red-brown seed coats to differ from brown by the presence of 3-0-galactosides of cyanidin and delphini-din (Fig. 4.2). An enzyme with minor 3-0-galactosyltransferase activity has been identified [26], but it remains to be determined whether genetic differences in this gene account for red-brown versus brown seed coat color. 

Valade, A. Urban, D. Beau, J.-M. Two galactosyltransferases selection of different binders from the same uridine-based dynamic combinatorial hbrary. J. Comb. Chem. 2007, 9,1. ... 

Figure 39 Schematic of the active site of /3-1,3-galactosyltransferase from Bos taurus.

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