Galactosyltransferase interactions

O Keefe, E.T., Mordick, T., and Bell, J.E. (1980) Bovine galactosyltransferase Interaction with a-lactalbu-min and the role of a-lactalbumin in lactose synthase. Biochemistry 19, 4962-4966. 

Galactosyltransferase is only one member within the broader classification of glycosyltransferases that have been found on cell surfaces these are involved in contact-mediated cell interactions in general, but more specifically are involved in fertilization, morphogenesis, hemostasis, and cell migration. This topic has been well reviewed by Pierce et al. (1980). 

C. Interactions of Galactosyltransferase and a-LactaUmmin in the Lactose Synthase System... 

A considerable effort has been expended, in several laboratories, on the kinetics of interactions involving galactosyltransferase. The results of Morrison and Ebner (1971a) were not in accord with any mechanism whereby substrates might add in any order, either wholly or partially random. The simplest mechanism, consistent with their kinetic data, was an ordered mechanism, whereby additions to galactosyltransferase proceed in the order Mn(II), UDP-galactose, and 7V-acetylglucosamine. 

The interactions of immobilized oc-lactalbumin with o-galactosyltransferase and with anti-(oc-lactalbumin) antibodies have been studied by fluorometry. ... 

Powell, J. T., and Brew, K., 1976, A comparison of the interactions of galactosyltransferase with a glycoprotein substrate (ovalbumin) and with d-lactalbumin, /. Biol. Chem. 251 3653. 

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