Galactose oxidase analysis

Table III). Atomic absorption analysis shows that the copper is still present in the enzyme. These two facts coupled with the 600-nm region intensity increase might be interpreted to suggest that a stereochemical distortion accompanies tryptophan oxidation in galactose oxidase. For example, a saddling of the planar environment would be expected to lower the reduction potential of the Cu(II)-Cu(I) couple. 

McPherson, M. J., Ogel, Z. B., Stevens, C., Yadav, K. D. S., Keen, J. N., and Knowles, P. F., Galactose oxidase of Dactylium dendroides gene cloning and sequence analysis, 1992, J. Biol. Chem. 267 8146n8152. 

Different types of leukaemic cells have been surface-labelled by sequential treatment with neuraminidase, D-galactose oxidase, and sodium borotritide. Analysis of the surface glycoprotein profile by polyacrylamide gel electrophoresis has provided a method for the classification of leukaemic cells according to cell type and the stage of differentiation. 

A method for the analysis of a two-substrate enzymic reaction taking place inside a porous support has been described. The resulting equations were applied to D-galactose-oxidase-catalysed oxidation of D-galactose when the enzyme is immobilized on porous-glass particles. The physical constants of the system were obtained from theoretical predictions, while the concentration of the... 

There are many oxidases used in clinical and analytical chemistry for the specific determination of constituents of complex samples, based on the generation of hydrogen peroxide by the action of an aerobic oxidase on its substrate. Cholesterol, glucose, galactose and many other analytes are determined that way. These analyses are also important in food analysis. But the volume of oxidases consumed in food processing is primarily based on the protection afforded the food by the removal of either oxygen or the other substrate acted upon. 

---