Funnel interface material

Sometimes some material comes out, or will not dissolve in the two liquid layers, and hangs in there in the interface. It may be that there s not enough liquid to dissolve this material. One cure is to add more fresh solventofone layer or the other. The solid may dissolve. Ifthere snoroom to add more, you may have to remove both (yes, both) layers from the funnel, and try to dissolve this solid in either of the solvents. It can be confusing. If the material does not redissolve, then it is a new compound and should be saved for analysis. You should see your instructor for that one. 

It would seem unlikely that the total volume of water in a high flux system, directed by a high-surface-area funnel, could be infiltrated through the much smaller surface area of the gate and aquifer material interface that contacts the downgradient side of the gate. 

Acidification of aqueous concentrates and extracts to pH near 1 is the standard procedure to precipitate humic from fulvic acid, and this procedure also has been applied to aquatic humic substances (Thurman and Malcolm, 1981). Aquatic humic substances that interact significantly with metal ions can be precipitated from water by addition of lead(Il) nitrate (Klocking and Mucke, 1969). Co-precipitation of aquatic humic materials with aluminum, copper, iron, and magnesium hydroxides has been used to recover aquatic humic substances from various types of water (Jeffrey and Hood, 1958 Williams and Zirino, 1964 Zeichmann, 1976). Humic acids can also be precipitated from an unconcentrated water sample by adding acetic acid and isoamyl alcohol to a sample contained in a separatory funnel, and after shaking, humic acid precipitates at the alcohol-water interface (Martin and Pierce, 1971). Precipitation methods are among the crudest of fractionation methods... 

---