Fungi inoculation

Morgan, P., Lee, S. A.,Lewis,S.T., Sheppard, A. N. Watkinson, R.J. (1993). Growth and biodegradation by white-rot fungi inoculated into soil. Soil Biology and Biochemistry, 25, 279-87. 

Andersson BE, S Lundstedt, K Tornberg, Y Schniirer, LG Oberg, B Mattiasson (2003) Incomplete degradation of polycyclic aromatic hydrocarbons in soil inoculated with wood-rotting fungi and their effect on the indigenous soil bacteria. Environ Toxicol Chem 22 1238-1243. 

D. Werner, S. Bernard, E. Gorge, A. Jacobi, R. Rape, K. Kosch, M. Pamiske, S. Schenk, P. Schmidt, and W. Streit, Competitiveness and communication for effective inoculation by Rhizohium, Bradyrhizohium and vesicular-arbuscular mycorrhiza fungi. Experientia. 50 884 (1994). 

Vierheilig, H., H. Gagnon et al. (2000). Accumulation of cyclohexenone derivatives in barley, wheat and maize roots in response to inoculation with different arbuscular mycorrhizal fungi. Mycorrhiza 9(5) 291-293. 

Clinical specimens obtained for the recovery of dematiaceous fungi usually do not require extensive processing. If aspirated specimens contain a substantial amount of purulent material, this can be dissolved with N-acetyl-L-cysteine without sodium hydroxide. Tissue specimens and biopsy material should be homogenized in a tissue homogenizer after highly suspicious areas consisting of necrotic, purulent, or caseous material are selectively examined microscopically and inoculated onto isolation media. 

Fungal infections are rarely transmitted directly from person to person. Fungi are derived from the commensal flora of the patient or from animal and innate sources in the environment, and are inoculated by (micro)trauma, ingestion or inhalation of spores. The incidence of invasive fungal infections among hospitalized patients has increased primarily due to the introduction of medical interventions that compromise the natural defenses of the patients. 

Inoculate the surface of tryptone soya agar slant for bacteria and Sabouraud dextrose agar slant for fungi from recently revived stock culture of each of the test microorganisms. 

Growth usually involves elongation of hyphae at their tips or apices. In most fungi every part of the mycelium is capable of inoculation and a small piece of mycelium is sufficient to produce a new thallus (Fig. 5.7). 

Fungal Decay of Woods. Blocks of sweet gum and southern pine sapwood were inoculated with test fungi by the standard soil-block method (7). The test fungi were the brown rots Poria monticolla and Lentinus lepidius and the white rot Polyporus versicolor. As a control, one block of pine and one block of gum were left in the sterilized soil-block chambers in which the fungus had been started on feeder blocks and then sterilized. 

---