Functional proteomics chemical proteomic strategy

Activity-based protein profiling (ABPP) is a chemical proteomic strategy in which active-site-directed covalent probes are used to profile the functional states of enzymes in complex proteomes. Activity-based probes (ABPs) can distinguish active enzymes from their inactive zymogens or inhibitor-bound forms. They contain a reactive group intended to modify enzyme active sites covalently and a reporter group (typically rhodamine or biotin) that assists in detection and identification of protein targets. 

The goal of this chapter is to detail the need for such activity-based methods, and to describe the development and application of ABPP by highlighting several studies that have established the utility of this chemical proteomic method as a powerful strategy for the discovery and functional analysis of complex biological proteomes, as well as their individual constituents. 

---