Fucosyltransferase

DeBose-Boyd, R.A., Nyame, A.K. and Cummings, R.D. (1998) Molecular cloning and characterization of an al,3 fucosyltransferase, CEFT-1 from Caenorhabditis elegans. Glycobiology 8, 905-917. 

Figure 14. a) The putative transition states for the fucosidase- and fucosyltransferase-catalyzed reactions, b) Iminocyclitol transition state analogs which may be used... 

R. Wischnat, R. Martin, C.-H. Wong, Synthesis of a New Class of N-Linked Lewis and LacNAc Analogues as Potential Inhibitors of Human Fucosyltransferases A General Method for the Incorporation of an Iminocyclitol as a Transition-State Mimetic of the Donor Sugar to the Acceptor , J. Org. Chem 1997, 63, 8361-8365. 

This class of enzymes includes galactoside 3(4)-L-fucosyl-transferase, [EC 2.4.1.65] glycoprotein 6-a-L-fucosyl-transferase [EC 2.4.1.68], galactoside 2-ce-L-fucosyltran-sferase [EC 2.4.1.69], and galactoside 3-fucosyltransferase [EC 2.4.1.152]. 

Galactoside 2-L-fucosyltransferase [EC 2.4.1.69], also known as blood group H a-2-fucosyltransferase, catalyzes the reaction of GDP-L-fucose with /8-D-galacto-syl-R to produce GDP and a-L-fucosyl-l,2-/3-D-galac-tosyl-R. Lactose can also act as the acceptor substrate. 

GALACTOSIDE FUCOSYLTRANSFERASES GALACTOSIDE SIALYLTRANSFERASES /3-D-Galactosyl-1,4-y3-D-glucosylceramide, SIALYLTRANSFERASE "GAMESS,"... 

Fucosyltransferase in pea microsomal preparations also fucosylated exogenously-added xyloglucan acceptors in addition to endogenous primers... 

Figure 7.29 Mechanism and inhibition of human fucosyltransferases by fluoro-GDP-fucoses. ...

Fucosyltransferases (glycosyltransferases) promote the transfer of fucose from GDP-fucose onto a saccharidic acceptor. This transfer occurs with inversion of the configuration of the anomeric carbon. The inhibition has been studied with fluorinated substrates. GDP-2-fluoro-2-deoxyfucoses (GDP-2F-fucose) and GDP-6-fluoro-6-deoxyfucoses (GDP-6F-fucose) are competitive inhibitors. The values are close to or less than that of (Figure 1.29)P The very close values of GDP-2F-fucose... 

The iminoaltritol (74) as well as the iminogulitol (77) could also be shown to be moderate inhibitors of a recombinant human a-l,3-fucosyltransferase. 

In 2004, Strasser et al. generated a xylT knockout mutant that no longer produced [3(1,2)-linked xylosyltransferase, and nfucTA andfucTB double knockout mutant that was unable to produce a(l,3)-fucosyltransferase, in... 

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