FTMS applications

The LTQ-FT mass spectrometer was introduced in late 2003 and, as expected, the main application discussed in the literature is for the analysis of proteins and peptides (Johnson et al., 2004 Syka et al., 2004). A recent book chapter (van der Greef et al., 2004) and a review article (Brown et al., 2005) discussed the application of the LTQ-FT to metabolomics. FTMS applications to dmg metabolism are still very new and dmg discovery research laboratories which have recently purchased the instmment are still in the process of developing and validating methods and approaches. A recent publication describes the depth and flexibility of the experimental setup utilizing accurate mass data-dependent exclusion MS" measurements with a LTQ-FT (Tozuka et al., 2005). We have reported several integrated approaches for determination of metabolic stability, characterization of metabolites and metabolic... 

It is clear that laser desorption Fourier transform mass spectrometry has significant potential as a polymer analysis tool. It works especially well for polar polymers and additives witii molecular weights lower than 10000 Da. AAftth proper attention to analytical details, it is a highly accurate and quite specific way to characterize tiiese substances and can provide unrivaled mass resolution. As is obvious from this chapter, the application of LD-FTMS to polymer analytical problems is an active area of investigation in many laboratories. AAftth the continuing proliferation of commercial FTMS instruments, LD-FTMS applications of polymer analysis should be expected to expand for the foreseeable future. There is no question tiiat tiiis method provides a versatile alternative to tire numerous less specific analytical methods employed to date. 

The utility of MALDI-FTMS analysis for use in chemotaxonomic applications has been established, but this method can be applied to other areas of interest, such as biomedical and environmental analyses. A common method used by biochemists and biologists today is recombinant overexpression of proteins using bacterial whole cells in cases where large quantity of a protein is desired. The main method presently used to determine if the overexpression was successful is the use of SDS-PAGE (sodium dodecylsulfate-poly acrylamide gel... 

Sanders, M., Shipkova, P. A., Zhang, H., and Warrack, B. M. (2006). Utility of the hybrid LTQ-FTMS for drug metabolism applications. Curr. Drug Metab. 7 547-555. 

Although both hybrid LTQ-FTMS instruments find applications in the dmg metabolism area, the LTQ-Qrbitrap, with its simplified maintenance, lower cost, and in most cases better sensitivity at low masses, is generally more applicable to small-molecule analysis. The LTQ-FTICR, with the advantage of higher resolution and mass accuracy, covers a wider range of applications, from small molecules to peptides and proteins, where most advantages are found in the analysis of large biomolecules. 

McDonald, L. A., Barbieri, L. R., Carter, G. T., Krappa, G., Feng, X., Lotvin, J. A., and Siegel, M. M. (2003). FTMS structure elucidation of natural products Application to muraymycin antibiotics using ESI multi-CHEF SORI-CID FTMS(n), the top-down/bottom-up approach, and HPLC ESI capillary-skimmer CID FTMS. Anal. Chem. 75 2730-2739. 

Application of LTQ-FTMS to metabonomics profiling. In Proceedings of the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, Nashville, TN. 

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