5-FSA

Fractal Dimensions of the Profiles h(x) at Various Morphological Amplitudes rj in h(x) = 77/wsCv) Determined by the Current Transient Technique (2nd Column) and the Triangulation Method (3rd Column). Here, /ws(-v) Means the Weierstrass Function with a Self-Affine Fractal Dimension t/Fsa = 1.5 ... 

Figure 14.8. Enzymatically-amplified TR-FIA ofa-fetoprotein (AFP), using streptavidin-alkaline phosphatase conjugate (SA-ALP) and 5-lluorosalicyl phosphate (FSAP) to generate 5 fluorosalicylic acid (FSA). The FSA comhines with terbium-EDTA to form a bright, fluorescent complex. (Adapted from Ref. 83 with permission.) (01992, American Chemical Society).

Protein content Bradford s test was performed to determine the protein content (mg g ) of the lyophilisates. A sample of the lyophilisate (2.5 mg) was dissolved in glycylglycine (Gly-Gly) buffer (1.5 mL). An ahquot of this solution (20 gL) was diluted with glycylglycine (Gly-Gly) buffer (30 gL) and Bradford s solution (950 gL) was added. After 5 min, the absorbance was measured at 595 nm. The concentration of FSA is calculated from the interpolation of the calibration curve of bovine serum albumin (BSA) 437 mg total protein/gram of lyophihsate. 

Abbreviations L, see Table 111 bdhe = V,V-bis(2-diethylamimKthyf)-2-hydroxyethylamine DBA= l,J-diphenyl-l,3,5-pentanetrionato dhph = 1,4-dibydrazinophthalazine H4(fsa)2en = N.N -bis(2-hydroxy-3-carboxybenzylidene)-1,2-diaminoethane (prp)aen = JV,W-ethylenebis[2-hydroxypropiophenone iminato]. 

Fig. 10. (A) Structural model of rhodopsin showing the location of cysteine substitution mutants (136-155) in C2 and adjacent sequences in TM3 and TM4. (B) fsa values computed from the crystal structure for both A and B molecules (upper panel), and 5 1 (the inverse central linewidth) (bottom panel) for R1 at each site. The shaded vertical bars mark residues in TM3 and TM4.

Figure 5 Perspective view of CuVO(fsa)2en-MeOH. The copper site is on the left side of the cage, the vanadyl to the right. The methanol molecule is not shown for clarity ...

As we saw in Chapter 5, dioxin (or TCDD) is a chemical contaminant derived from industrial processes that occurs in the environment and which can be found in very small amounts in some kinds of food (for example, oily fish). How do regulatory authorities like the Food and Drug Administration (FDA) in the USA or the Food Standards Agency (FSA) in the UK decide on a value for the tolerable daily intake for this chemical ... 

Fig. 5. Overlap density gCuVO in CuVO(fsa)2en CH3OH (top) and [(tmen)(H20)Cu(C204)V0(H20)(C204)] (bottom)...

H4(fsa)2(en)=A, -(2-hydroxy-3-carboxybenzilidene) ethy-lenediamine (see Figure 5). The copper (II) and vanadium (IV) sites, the oxygen atom of the vanadyl group and the methanol oxygen atom weakly bonded to the copper atom (not shown) are very close to forming a mirror plane perpendicular to the plane of the macrocycle. The unpaired copper electron therefore has close to a pure xy-type wavefunc-tion transforming as a" while the unpaired electron around the vanadium is described by a magnetic orbital... 

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