Fructosyltransferase purification

S. A. van Hijum, K. Bonting, M. J. van der Maarel, and L. Dijkhuizen, Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced, FEMS Microbiol. Lett., 205 (2001) 323-328. 

Koops, A.J. and Jonker, H.H., Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus Columbia. 1. Fructan fructan 1-fructosyltransferase, J. Exp. Bot., 45, 1623-1631, 1994. 

Luscher, M., Purification and characterization of fructamfructan fructosyltransferase from Jerusalem artichoke, New Phytologist, 123, 717-724, 1993. 

Pectinex Ultra SP-L is a commercial enzyme preparation from Aspergillus acu-leatus that is used in the food industry for reducing viscosity in fruit juice processing. It contains different pectinolytic and cellulolytic enzymes [29]. In addition, the existence of fructosyltransferase activity in Pectinex Ultra SP-L has been reported by several authors [30-32]. In recent years, we have investigated the purification, characterization, and application of the fructosyltransferase from A. aadeatus contained in this commercial preparation [33]. 

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