Purification of Fructosyltransferase in Pectinex Ultra SP

Pectinex Ultra SP-L is a commercial enzyme preparation from Aspergillus acu-leatus that is used in the food industry for reducing viscosity in fruit juice processing. It contains different pectinolytic and cellulolytic enzymes [29]. In addition, the existence of fructosyltransferase activity in Pectinex Ultra SP-L has been reported by several authors [30-32]. In recent years, we have investigated the purification, characterization, and application of the fructosyltransferase from A. aadeatus contained in this commercial preparation [33]. 

Aspergillus aculeatus Fructosyltransferase from 3 LDTTA P P PTN LSTLPNNTLF 22 

Aspergillus niger ATCC 20611 Invertase from Aspergillus niger B50 6 DYNVAPP-NLSTLPNGSLF 23 

2 on the basis of relative mobiUty on a 4% polyacrylamide gel under denaturing conditions. 

Fructosyltransferases and P-fmctofuranosidases from various sources show substantial differences in their molecular properties (subunit molecular weight. 

---