Fretting sites

Specific applications of FRET and FRET include observation of myosin movement (Fig. 19-14),157 measurement of distances between binding sites on tubulin,161 determining stoichiometry of subunit assembly in a y-aminobutyrate receptor of brain,162 association of proteins in peroxisomes,160 study of hydridization of deoxyribonucleotides,163 verifying the handedness of various forms of DNA,164 and other studies of DNA and RNA.164a b... 

Bacchiocchi, C., and Lehrer, S. S. (2002). Ca(2+) -induced movement of tropomyosin in skeletal muscle thin filaments observed by multi-site FRET. Biophys.J. 82,1524—1536. 

The elucidation of the structure, dynamics and self assembly of biopolymers has been the subject of many experimental, theoretical and computational studies over the last several decades. [1, 2] More recently, powerful singlemolecule (SM) techniques have emerged which make it possible to explore those questions with an unprecedented level of detail. [3-55] SM fluorescence resonance energy transfer (FRET), [56-60] in particular, has been established as a unique probe of conformational structure and dynamics. [26-55] In those SM-FRET experiments, one measures the efficiency of energy transfer between a donor dye molecule and an acceptor dye molecule, which label specific sites of a macromolecule. The rate constant for FRET from donor to acceptor is assumed to be given by the Forster theory, namely [59,61-64]... 

We have developed the splinted RNA ligation procedure outlined in this chapter to generate site-specifically dye-labeled telomerase RNA constructs. These modified telomerase RNA constructs may be used to characterize dynamic RNA structural properties using Forster resonance energy transfer (FRET) (Stone et al., 2007). Our laboratory specializes in single molecule FRET measurements, which facilitates the direct observation of transient RNA structural states. The details of single molecule FRET... 

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