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Freeze drying pathogenic materials

Standard procedures were followed for isolation of the toxic principles from mycelium of FA 120. A methylene chloride extract of the freeze-dried hyphae was initially partitioned between hexane and aqueous methanol to separate lipids from more polar material. Bio-assay-monitored chromatographic fractionation of the hexane-soluble material led to the isolation of a fraction (ca. 5% of the hyphal weight) which could account for much of the toxicity of the hyphae of FA 120 to spruce budworm larvae. The spectroscopic and chemical properties of this material were characteristic of the enniatins, a group of cyclic hexadepsipeptide ionophore antibiotics produced by several plant pathogenic Fusarium species, including F. lateritium... [Pg.114]

See other pages where Freeze drying pathogenic materials is mentioned: [Pg.124]    [Pg.127]    [Pg.130]    [Pg.132]    [Pg.361]    [Pg.365]    [Pg.368]    [Pg.371]    [Pg.360]    [Pg.364]    [Pg.367]    [Pg.370]    [Pg.117]    [Pg.120]    [Pg.352]    [Pg.356]    [Pg.351]    [Pg.355]    [Pg.181]    [Pg.209]    [Pg.11]    [Pg.269]    [Pg.1199]    [Pg.702]    [Pg.1446]   
See also in sourсe #XX -- [ Pg.181 ]



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