Fragmentation-derived sequence tags

The second approach is the tandem mass spectrometric method (Wilm et ah, 1996 Link et ah, 1999 Yates, 2000). This method relies on fragmentation of individual peptides in the tryptic peptide mixture to gain sequence information. Its main advantage is that sequence information derived from several peptides is much more specific for the identification of a protein than a list of peptide masses. The sequence data can be used to search not only protein sequence databases but also nucleotide databases such as expressed sequence tag (EST) databases and, more recently, even... 

The novel cDNA fragments will serve as useful landmarks known as sequence-tagged sites (STSs) along the different human chromosomal locations they are derived from, and will aid in the human genome project discussed below (Adams et al, 1991). In addition, the unique ESTs will aid isolation of the corresponding full-length cDNAs, and identification of novel proteins, some of which may have therapeutic... 

D.L. Tabb, A. Saraf, J.R. Yates, III, GutenTag High-throughput sequence tagging via an empirically derived fragmentation model. Anal. Chem., 75 (2003) 6415. 

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