High throughput sequencing

Their proprietary technology package includes FLEX and MicroFLEX for library construction, and is based on high-throughput sequencing, tracking and analysis systems. 

More recently, high-resolution chromatographic techniques (particularly reverse-phase and ion exchanged-based HPLC) have been applied in the separation of proteome proteins and high-resolution mass spectrometry is being employed to aid high-throughput sequence determination. 

COMPARISON OF MICROARRAYS WITH HIGH-THROUGHPUT SEQUENCING... 

EST Expressed sequence tag, a short (100s bp) partial nucleotide sequence from a full-length or nearly full-length cDNA. These sequences are usually from the 5 or 3 ends of directionally cloned inserts as part of high-throughput sequencing projects. The partial cDNA sequence provides annotation by similarity searches and the putative identification of function. 

Joo SH, Xiao Q, Ling Y, Gopishetty B, Pei D. High-throughput sequence determination of cyclic peptide library members by partial edman degradation/mass spectrometry. J. Am. Chem. Soc. 

D.L. Tabb, A. Saraf, J.R. Yates, III, GutenTag High-throughput sequence tagging via an empirically derived fragmentation model. Anal. Chem., 75 (2003) 6415. 

The data-controlled automation of LC/MS could also make it possible to perform the high-throughput sequencing of peptides [34] or oligonucleotides from pooled libraries. A constraint of this approach is vast amount of data that must be interpreted. Software-supported spectral interpretation must be improved before this approach can become practical. 

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