Fractionation, of polysaccharides

In addition to classical reverse phase separation of peptides on octadecyl derivatized silica monoliths, sugars and peptides as well as proteins and nucleosides have been analyzed on a 20-cm-long silica-based poly(acrylic acid) column (ID. 200 pm), employing HILIC and weak cation-exchange chromatography, respectively [194]. Furthermore, HILIC fractionation of polysaccharides delivered remarkable and promising results [84,194]. 

Chen R, meng F, Liu Z, Chen R, Zhang M. Antitumor activities of different fractions of polysaccharide purified from Ornithogalum caudatum Ait. Carbohydrate Polymers 2010 80 845-851. 

In particular with respect to the fact that H2O is an integral component of the morphology and functionality of polysaccharide-containing compartments, the dependence of material characteristics on the de facto concentration or volume fraction of polysaccharides... 

Aspinall, G.O. R. Begbie A. Hamilton J.N.C. Whyte. Polysaccharides of soy-beans. Part III. Extraction and fractionation of polysaccharides from cotyledon meal. /. Chem. Soc. 1967c, 1065-1070. 

P. Mischnick and R. Adden, Fractionation of polysaccharide derivatives and subsequent analysis to differentiate heterogeneities on various hierarchical levels, Macromol. Symp., 262 (2008) 1-7. 

Yu et al (15) have studied reaction of solid state enzymes on functional properties of psyllium seed husk and observed friat modified psyllium showed improved gelling properties, unproved dispersing effect and more desirable sensory properties and decreased water absorbing capacity. Modified psyllium still contains soluble and insoluble fiber with fewer undesirable properties. Kennedy et al (14) and Fischer et al (13) have developed an efficient, reproducible process for alkaline extraction and fractionation of polysaccharide from husk which can be useful for further biological studies. 

Stoehr T, Petzold K, Wolf B.A., Klemm D., Continuous polymer fractionation of polysaccharides using highly substituted trimethylsUylcellulose, Macramo/. Chem. Phys., 199, 1998,1895-1900. 

Microcrystalline cellulose treated in turn with epichlorohydrin and di-N-ethyl-2,3-epoxypropylamine afforded an 0-(3-diethylamino-2-hydroxypropyl) derivative that can be used in the fractionation of polysaccharides. ... 

Starch cross-linked with epichlorohydrin has been used as an aiTlnity matrix in the purification of a-amylases and, following reaction with JViV-diethy 1-2,3-epoxy propylamine, in the fractionation of polysaccharides. ... 

Two new ion-exchange polysaccharides have been developed for use in the fractionation of polysaccharides and other biopolymers. Pectins from the bark of Salix alba were separated on columns of 0-(3-diethylamino-2-hydroxy-propyl)starch (DEAHP-starch) into six components differing in uronic acid content and in molecular size. The corresponding cellulose derivative (DEAHP-cellulose) was used in the fractionation of glucomannans from the same source. The methodology and the instrumentation used in ion-exchange chromatography... 

Starch cross-linked with epichlorohydrin reacted with AW-diethyl-2,3-epoxy-propylamine to form an ion-exchange material that is suitable for the fractionation of polysaccharides and other biopolymers. Starches cross-linked with either epichlorohydrin or phosphorus oxychloride adsorb ot-amylase. ... 

The Polysaccharides, Vol. 1 (G. O. Aspinall, ed.) Academic, New York (1982). Chap. 1, General introduction Chap. 2, Isolation and fractionation of polysaccharides Chap. 5, Shapes and interaction of carbohydrate chains Chap. 6, Immunology of polysaccharides. 

Aspinall, G. O., and J. A. Molloy Pectic Substances from Lucerne (Medicago sativa). Part III. Fractionation of Polysaccharides Extracted with Water. J. Chem. Soc. (C), 2994 (1968). 

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