Formate assay and, III

Hydroperoxides cause oxidation of Fe(II) ions to Fe(III) in acid solution. In a modification of the FOX assay (Section III.B.2.a) the oxidized ions form a blue-purple 1 1 complex with Xylenol Orange (71) that can be measured at 560 nm"" """. However, it has been found that formation of a 2 1 complex is also possible and some corrections need be made to obtain a good correlation between this method and POV obtained by the AOCS official method"" . A modification of the FOX method proposed for determination of POV in beef, chicken, fish, butter and vegetable products correlates well with standard iodometric and FOX assays"". ... 

Fluid delivery into a microfluidic cell culture chip has three primary purposes (i) cell seeding, (ii) perfusion with culture medium, and (iii) introduction of cellular effectors during an assay. In the simplest format, a system has one inlet, which is used for all three purposes (Fig. 4a). If the fluidic path that is used for cell seeding is long and has multiple branches such as the flow equalizer in Fig. 4a, premature sedimentation of cells along the fluidic path is easily the consequence. In order to alleviate such a problem, a dedicated inlet for cell seeding is recommended such a system is shown in Fig. 7a [7]. The system has, however, a common inlet for perfusion with culture medium and introduction of cellular effectors. 

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