Formaldehyde calibration curve preparation

Alternatively, air drawn through a PTFE membrane followed by sodium bisulfite solution in impingers impinger solution treated with chromotropic acid and H2S04 color developed due to formation of a derivative of formaldehyde absorbance measured by a spectrophotometer at 580 nm a standard calibration curve prepared from formaldehyde standard solutions for quantitation (NIOSH Method 3500,1989) recommended air flow 500 mL/min sample volume 50 L. 

Calibration standards prepared from formaldehyde (formalin) stock solutions each standard injected into 120 mg portions coated-adsorbent derivative desorbed into toluene and analyzed by GC-FID as above calibration curve constmcted (plotting area/height response against concentrations) for quantitation. 

Preparation of calibration curve for formaldehyde analysis. The calibration curve for thiazolidine (formaldehyde derivative) was prepared with N-methylacetamide as an internal standard. 

Test solution (a) in 200 dilution of the vaccine to be examined with R is prepared as described in the pharmacopoea. Solutions containing 0.25 g/1, 0.50 g/1,1.00 g/1, and 2.00 g/1 of CH2O by dilution of formaldehyde solution R with R are prepared. To 0.5 ml of the test solution and of each of the reference solutions in test tubes, 5.0 ml of a freshly prepared 0.5 g/1 solution of methylbenzoethiazolone hydrazone hydrochloride R is added. Tubes are closed, shaken, and allowed to stand for 60 min. One ml of ferric chloride-sulfamic acid reagent R is added and allowed to stand for 15 min. The absorbance of the solutions is measured at 628 nm, and the content of formaldehyde in the vaccine to be examined is calculated from the calibration curve established using the reference solutions. The test is invalid if the correlation coefficient (r) of the calibration curve is less than 0.97. 

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