For oligonucleotide synthesi

This substantial group was developed as a fluorescent, acid-labile protective group for oligonucleotide synthesis. It has properties very similar to those of the DMTr group except that it can be detected down to 10 M on TLC plates with 360-nm ultraviolet light. 

The mechanism of the Li-Nicolaou experiment shows the manifold possibilities for oligonucleotide synthesis. However, this replication system is limited to special... 

U. Maskos, E.M. Southern, Oligonucleotide Hybridizations on Glass Supports A Novel Linker for Oligonucleotide Synthesis and Hybridization Properties of Ohgonucleotides Synthesised In Situ , Nucl. Acids Res., 20(7), 1679-1684 (1992). 

The phosphoramidite derivative of N-nitrothymidine (44) has been synthesised and found suitable for oligonucleotide synthesis using a standard phosphite triester solid phase approach. The N-nitrothymidine residues could be converted into a range of N -modified thymidines by reaction with primary alkyl amines. Phosphoramidite derivatives of 4-nitroindazole N and N -(2 -deoxy-p-D-ribofuranosides) (45, 46) have been synthesised, their base pairing properties investigated and found to show ambiguous base pairing. Seela has also reported the syntheses of the phosphoramidite derivatives of 8-aza-7-adenine... 

Oligonucleotide synthesis. - 1.1.1 DNA Synthesis. There have been few publications on improvements to DNA synthesis, and the main area of development has been in the development of modified oligonucleotides (ODNs). There have, however, been some improvements in the use of supports, reagents and protecting groups. Two new thymidine modified solid supports have been prepared suitable for oligonucleotide synthesis in which the support is attached via the thymine N3 position. These modified supports allow for ODN synthesis... 

Papers which report the synthesis of dinucleoside monophosphates or their analogues as model studies for oligonucleotide synthesis are covered in section 4. 

A universal support for oligonucleotide synthesis has been described (136). The support was synthesised from 1,4-anhydro-D-ribitol. ... 

Maskos U, Southern EM (1992) Oligonucleotide hybridizations on glass supports a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ. Nucleic Acids Res 20(7) 1679-1684... 

Synthesis of a diene (285) (Scheme 63) [305] Polymer-supported triphe-nyl[(lE)-2-piperazin-l-ylprop-l-enyl]phosphonium bromide as described above was (0.16 mmol) suspended in a suitable inert solvent which can form an azeotropic mixture with water, and residual amounts of moisture were removed upon co-evaporation. (The authors of ref [305] suggest using highly toxic benzene, which they apparently partially removed at ambient pressure with a stream of Ar. We recommend washing the resin in a Schlenk-frit sealable reactor block with anhydrous MeCN (for oligonucleotide synthesis). 

This group was developed as a fluorescent silyl protective group for oligonucleotide synthesis. It has excitation and emission wavelengths of 346 nm and 390 nm, respectively, which are outside the range of the DNA-damaging wavelength of 254-260 nm. It is prepared from the in situ prepared silyl chloride. It is stable to 0.01 M HCl and 30% ammonia. It is cleaved with 0.1 M TBAF in 3 min at rt. ... 

This is one of the more commonly used groups for phosphate protection, especially for oligonucleotide synthesis, but its base sensitivity can be a problem in some circumstances. Upon deprotection, acrylonitrile is released, which can result in byproduct formation by alkylating nucleophilic substituents. 

Polypropylene also has favourable physical and chemical properties. Its surface is aminated by plasma discharge in the presence of anhydrous ammonia [2]. The free amine groups (-NH2) thus produced on its surface are also a substrate for oligonucleotide synthesis with standard phosphoramidite chemistry. 

Scheme 19.2 Methods for polymer-supported oligonucleotide synthesis. Dinudeoside phosphate formation in the common methods for oligonucleotide synthesis phosphodiester (I), phosphotriester (II), phosphite triester (III) and H-phosphonate (IV).

Todd uncovered the potential of nucleoside H-phosphonates for oligonucleotide synthesis in the very early days of nucleic acid chemistry [15], However, this knowledge laid dormant for almost three decades, while other methods flourished. 

Uncross-linked polystyrene grafted onto polytetrafluoroethylene (PTFE, Teflon) was adopted for oligonucleotide synthesis according to the phosphodiester scheme by Potapov et al. [167] To produce their support, the authors y-irradiated PTFE... 

Many other polymers have been tried as supports for solid phase oligonucleotide synthesis. Even before the first use of polyacrylamide for oligonucleotide synthesis,... 

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