Follicle stimulating hormone hFSH

Sample material Serum or plasma (EDTA, oxalate or heparin). Citrate results in lower recovery. Serum or plasma can be stored for one week in the refrigerator at +4°C to +8°C. 

Specificity To determine the cross reactivity, pooled serum with an hFSH content of 0 or 15 U/1 was given additions of different quantities of hormones as listed below. The percentage cross reactivity is the quotient from the average hFSH concentration and the concentration of the cross-reacting substance that is required to achieve the same degree of binding to the antibody. 

Interferences Haemolytic (haemoglobin up to 6 g/1), lipaemic (triglycerides up to 1000 mg/dl or cholesterol up to 600 mg/dl) and icteric (bilirubin up to 100 mg/dl) samples do not exercise any relevant influence. However, the use of strongly haemolytic samples should be avoided. 

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Follitropin alpha, human follicle-stimulating hormone (hFSH), recombinant... 

Grasso P, Heindel JJ, Powell CJ, Reichert LE Jr (1993) Effects of mono(2-ethylhexyl)phthalate, a testicular toxicant, on follicle-stimulating hormone binding to membranes of cultured rat Sertoli cells. Biol Reprod 48 454-459 Ketelslegers JM, Catt KJ (1974) Receptor binding properties of 125-hFSH prepared by enzymatic iodination. J Clin Endocrin Metab 39 1159-1162... 

Scheme 9 Synthesis of the 3-subunit of human follicle stimulating hormone, P-hFSH(l-l 11]...

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