Focal adhesion formation

Kamioka H, Sugawara Y, Murshid SA, Ishihara Y, Honjo T, Takano-Yamamoto T. 2006. Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast implication of different focal adhesion formation. J Bone Miner Res 21 1012—21. 

Pesen D, Heinz WF, Werbin JL et al (2007) Electron beam patterning of fibronectin nanodots that support focal adhesion formation. Soft Matter 3 1280-1284... 

Woods A, Fongley RF, Tumova S, Couchman JR. Syndecan-4 binding to the high affinity heparin-binding domain of fibronectin drives focal adhesion formation in fibroblasts. Arch Biochem Biophys 2000 374 66-72. 

Pits Epithelial cells Focal adhesion formation... 

Keselowsky BG, Garcia AJ. Quantitative methods for analysis of integrin binding and focal adhesion formation on hiomaterial surfaces. Biomaterials 2005 26 413-8. 

Figure 4 illustrates some of the various phenomena that lead to cell migration. Some of these phenomena have been successfiilly formulated into mathematical models of cell migration and are marked with an asterisk in Figure 4. Table II lists some of the most significant models from the literature. These models account for such effects as the population dynamics, the individual cell movements, mechanical force balances between the cell and its substrate, intra-and extrace-llular signaling, and ligand-receptor binding. Other phenomena that are essential to cell migration, such as cytoskeletal rearrangement and focal adhesion formation have not been exhaustively modeled.

Promotes focal adhesion formation, ceU spreading, and matrix formation with fibroblasts... 

Mgat5 l breast tumor cells removed from the mice and placed in tissue culture showed impaired focal adhesion formation in low serum conditions (Figure 3). By activating c-Sre/FAK, Shc/RAS and PI3K, the PyMT protein contributes to the reorganization of cytoskeletal structures and turnover of focal-adhesion contacts... 

Maturation is associated with the formation of F-actin (actin filaments) stress fibres in the cell. Only an adequate maturation allows the potential cell proliferation and differentiation. When analysing the detailed molecular events in focal adhesion formation as a function of material surface properties, it becomes clear that the make-up of a focal adhesion can be highly variable, both in the ratios of the associated proteins present (such as vinculin and talin), as in the degree of phosphorylation in the different tyrosines within relevant proteins (Keselowsky and Garcia, 2005). It is still not clear how we can translate these observations to explain the cell behaviour differences, yet it is evident that mature focal adhesions are of pivotal importance for... 

Keselowsky, B., Garcia, A., 2005. Qnantitative methods for analysis of integrin hinding and focal adhesion formation on biomaterial snrfaces. Biomaterials 26 (4), 413-418. 

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