Fluorogenic chemical detection

The intent of this chapter is not to provide an exhaustive review of chemical- and biosensors and probes, but rather to offer a brief overview of existing optical techniques and an indepth analysis of near-infrared (NIR) fluorogenic probes and sensors for the detection of metal ions, solution pH, and biomolecules and to present some of the latest results. 

A method based on inhibition of ABAP-induced oxidation of a fluorogenic probe, 2, 7-dichlorodihydrofluorescein diacetate (H2-DCF-DA), commonly used for detection of production of reactive oxygen species, has been proposed (V2). Oxidation of the probe can be monitored either spectrophotometrically or fluori-metrically. A drawback of the method is the necessity of hydrolysis of the ester because only the deesterified from can be oxidized. The rate of hydrolysis depends on the hydrolytic activity of the samples measured. A modification consisting of chemical hydrolysis of H2-DCF-DA before measurement is impractical because 2,7 -dichlorodihydrofluorescein oxidizes rapidly. 

Royo S, Martmez-Manez R, Sancenon F, Costero AM, Parra M, Gil S. Chromogenic and fluorogenic reagents for chemical warfare nerve agents detection. Chem Commun 2007 4839-47. 

F. 2 a Schematic of templaled fluorogenic tetrazine ligations, b Schematic of template tetrazine-mediated transfer reaction for live cell miRNA detection, a Adapted from Ref. [78] with permission fiom Oxford University Press b Adapted with permission from [43]. Copyright (2014) American Chemical Society... 

Rational design of a chromo- and fluorogenic hybrid chemosensor material for the detection of long-Chain Carboxylates. Journal of the American Chemical Society, 127,184. 

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