Fluorescence titrations quantitative analysis

The outcome of many biochemical experiments can be expressed as simple descriptive statements, such as the desired band or peak was observed . In others, such as studies of the rates of processes or the affinity of a ligand for its target, the results need to be given in quantitative form as numerical values of one or more parameters. These quantitative results are derived by mathematical analysis of the raw experimental data. As an example, Figure 8-4 illustrates the time course of an enzymatic reaction. The raw data are the dependence of product concentration (the dependent variable, conventionally shown on the y-axis) on the time (the independent variable, conventionally on the x-axis). Another example is the result of the fluorescence titration of a protein with DNA shown in Figure 7-14. In this case, the independent variable is the concentration ratio of protein/DNA and the dependent variable is the observed fluorescence intensity. 

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