Fluorescence scattering theory

Fig. 27. Fluorescence date of Benner et al. (1980) for polystyrene microspheres that show fluorescence enhancement compared with elastic scattering theory.

Another type of intrinsic property is derived from the theory of light scattering in particles. The phenomenon of Raman and fluorescent scattering from molecules suspended in small dielectric particles exemplifies such prop-... 

Another complication in the quantitation of TIRF on cells is the effect of the membrane thickness itself on the profile of the evanescent wave. Reichert and Truskey<105) have calculated that, in theory, the thickness of the membrane should have a negligible effect on the fluorescence and that a simplified theory of three stratified layers (glass/water/cytoplasm) should be adequate. The theory approximates for simplicity that scattering plays a negligible role and that fluorescence intensity versus angle of observation and fluorescence lifetime are not functions of distance to the interface z. Experiments that... 

Benner et al. suspended in water relatively monodisperse polystyrene microspheres, which contained a fluorescent dye, and they recorded fluorescence spectra. The spheres had a nominal diameter of 9.92 /im (with a 2% standard deviation). Figure 27 presents one of their experimental fluorescence spectra (b) together with elastic scattering spectra computed for a diameter of 9.92 /an (a) and for 9.99 fim (c). The structure of the fluorescence sp>ectrum is not in agreement with the Mie theory computation for a 9.92/xm diameter sphere, but it is quite consistent with the result predicted for a 9.99/xm diameter sphere. 

Zhadin NN, Alfano RR. Correction of the internal absorption effect in fluorescence emission and excitation spectra from ahsorhing and highly scattering media theory and experiment. Journal of Biomedical Optics 1998, 3, 171-186. 

If h > 0, then helical assemblies do not form. There is an isodesmic polymerization from monomers to "disordered" supramolecular polymers that takes place around X l. The theory of Section 1 applies and the equivalent equilibrium constant obeys K = exp [. jy. Its value can be probed by means of radiation scattering, fluorescence decay, and UV absorption spectroscopy (Brunsveld et al., 2001 Jonkheijm et al., 2006). 

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