Fluorescence imaging system

Wokosin D L and White J G 1997 Optimization of the design of a multiple-photon excitation laser scanning fluorescence imaging system Proc. SPIE 2984 25-9... 

There are many sources of noise that affect the performance of fluorescence imaging systems. The sources of noise can be classified into [31] ... 

In this section, based on the methodology presented in the previous section, we describe multidimensional fluorescence imaging and its application to tracking cell responses. We developed the time- and spectrally-resolved fluorescence imaging system based on line illumination, which is capable of rapid acquisition of fluorescence intensities as a function of Em, x, and xy-positions. We applied it to the analysis of an induced plant defense response, that is, the accumulation of antimicrobial compounds or phytoalexins, in oat (Avena sativa). 

Fig. 1. Diagram of the optical path of a fluorescence-imaging system (ACAS 570 Interactive Laser Cytometer). This system consists of a tunable 5W Argon ion laser, an acousto-optic modulator (AOM) to control the duration and intensity of excitation, an inverted microscope with an X-Y scanning stage, a Z-axis stepper motor, and two photomultiplier (PMT) tubes. A computer performs command, control, and analytical routines.

Kim MS, Chen YR, Mehl PM (2001) Hyperspectral reflectance and fluorescence imaging system for food quality and safety. T ASAE 44 721-729... 

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