Fluorescence imaging, camera selection

Lakowicz, J. R. and Berndt, K. W. (1991). Lifetime-selective fluorescence imaging using an Rf phase-sensitive camera. Rev. Sci. Instrum. 62, 1727-34. 

Mixing was characterized by an optical microscope with an epifluorescence attachment [160], A 10 4 M fluorescein solution in sodium phosphate buffer (10 mM, pH 7, with trace of methanol) was applied. A filter cube was used for fitting the excitation and emission characteristics, allowing selective passages of the radiation. A CCD digital camera collected real-time fluorescence images. 

Fig. 4 Schematic of a typical experimental setup used to carry out p-PIV measurements. A pulsed Nd YAG laser or a continuous Hg arc lamp is used to excite fluorescently tagged polystyrene particles on an inverted epi-fluorescent microscope. A CCD camera is used to capture the particle images at regular well-defined time intervals. A filter cube is selected based on the fluorophore used (i.e., FITC for fluorescein or TRITC for rhodamine)...

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