Fluorescence highly amplified

There are however difficulties in running multiplex PCR, and it is difficult to differentiate PCR products of approximately the same size in a multiplex PCR or PCR-RFLP gel. However DNA microarrays do not have these problems, and have been used to serotype pathogenic strains and proved to be rapid, reliable and sensitive. The approach involves the immobilization of numerous oligonucleotide DNA probes on a solid support to which fluorescence-labeled amplified target DNA is hybridized, and is a powerful tool for the detection of pathogens by virtue of high throughput, speed and sensitivity. 

Xu H, Wu HP, Huang F, Song SP, Li WX, Cao Y, Fan CH (2005) Magnetically assisted DNA assays high selectivity using conjugated polymers for amplified fluorescent transduction. Nucleic Acids Res 33 e83... 

Chapter 9, Explosives Detection Based on Amplifying Fluorescence Polymers, describes a relatively new technology with very high sensitivity that has been applied successfully to several real-world search efforts. This is the sensing technology used in Chapters 6 and 7. 

Applications making use of the nonlinear absorption of dyes are passive Q-switching in solid-state lasers, pulse shaping, pulse intensity measurements of high-power ultrashort pulses, optical isolation between amplifier stages of high power solid-state lasers, and pulse width measurements of ultrashort pulses by the two-photon-fluorescence (TPF) method. 

Chemiluminescence can be greatly amplified by this process and it forms the basis of spectacular demonstrations of cold light. An example is the per-bydrolysis of ethanedioic (oxalic) esters with hydrogen peroxide in the presence of a fluorescent substance (Equation 28-12). The reaction is believed to pass through the highly unstable dioxacyclobutanedione, which dissociates into two moles of carbon dioxide with such exothermicity that electronic excitation occurs, as evident from the intense light produced in the presence of fluorescent dyes ... 

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