Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Fluorescence activated phosphate detection

Three enzymes play an active role in the metabolism of vitamin B6 in human erythrocytes. Pyridoxal kinase uses ATP to phosphorylate pyridoxine, pyri-doxamine, and pyridoxal. Pyridoxamine oxidase oxidizes pyridoxamine-5 -phosphate and pyridoxine-5 -phosphate to pyridoxal-5 -phosphate. The phosphatase activity produces pyridoxal from pyridoxal-5 -phosphate. The assay of the three enzymes required separation of the semicarbazone derivatives of pyridoxal-5 -phosphate and pyridoxal. The mobile phase used by Ubbink and Schnell (1988) contained 2.5% acetonitrile. Detection was by fluorescence. [Pg.373]

Enzyme-labelled fluorescence substrate (ELF 97 phosphate, also known as ELFP) used to detect phosphatase activity by staining the site of cleavage. [Pg.402]

Enzyme-labeled fluorescence (ELF) Various phosphate-labeled fluorescent dyes, ELF 97 Uses phosphatase-based signal amplification. Applicable to immunohisto-chemical and cytological staining, mRNA in situ hybridization, detection of endogenous phosphatase activity, and blot analyses. 34... [Pg.613]

See other pages where Fluorescence activated phosphate detection is mentioned: [Pg.61]    [Pg.556]    [Pg.389]    [Pg.389]    [Pg.203]    [Pg.308]    [Pg.87]    [Pg.36]    [Pg.3]    [Pg.91]    [Pg.340]    [Pg.367]    [Pg.269]    [Pg.278]    [Pg.451]    [Pg.33]    [Pg.125]    [Pg.8]    [Pg.91]    [Pg.102]    [Pg.192]    [Pg.82]    [Pg.91]    [Pg.542]    [Pg.554]    [Pg.1097]    [Pg.170]    [Pg.95]    [Pg.235]    [Pg.441]    [Pg.210]    [Pg.361]    [Pg.370]    [Pg.289]    [Pg.892]    [Pg.470]    [Pg.456]    [Pg.496]    [Pg.411]    [Pg.1548]    [Pg.1615]    [Pg.4924]    [Pg.511]    [Pg.582]    [Pg.160]    [Pg.73]    [Pg.80]    [Pg.614]   
See also in sourсe #XX -- [ Pg.389 ]

See also in sourсe #XX -- [ Pg.389 ]



SEARCH



Active detection

Fluorescence detection

Fluorescence-detected

Phosphate detection

© 2024 chempedia.info