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Fluorescein dichlorotriazine

Among the commercially available fluorescein derivatives that have been widely used are fluorescein isothiocyanate (FITC) 49, carboxyfluorescein (FAM) succini-midyl ester 50, and fluorescein dichlorotriazine (DTAF) 51. FITCs are the most commonly used fluorescein derivatives. They have been used to react with sulfhy-dryl [127], targeting reduced cysteine chains and especially amino groups in peptides or proteins [128]. [Pg.44]

MAA Methacrylic acid, EGDMA Ethylenglycoldimethacrylate, DAM Diethylaminoethylaminoethylmethacrylate, VPY4-Vinylpiridine DVB Divinylbenzene, CMMC 7-Carboxymethoxy-4-methylcoumarin, AA Allylamine, 4MU 4-Methylumbelliferone, OP A o-Phthalic dialdehyde + beta-mercaptoethanol, APBA 3-Aminophenylboronic acid, TBA 3-Thiopheneboronic acid, DTAF 5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein, FITC Fluorescein isothiocyanate... [Pg.145]

The first work in this field was probably that of Piletsky et al. [84] that described a competitive FILA for the analysis of triazine using the fluorescent derivative 5-[(4,6-dichlorotriazin-2-yl)amino]fluorescein. The fluorescence of the supernatant after incubation was proportional to the triazine concentration and the assay was selective to triazine over atrazine and simazine. The same fluorescent triazine derivative was applied to competitive assays using atrazine-imprinted films [70]. To this end an oxidative polymerization was performed in the presence of the template, the monomer(s) 3-thiopheneboronic acid (TBA) or mixtures of 3-amino-phenylboronic acid (APBA) and TBA (10 1) in ethanol-water (1 1 v/v) where the template is more soluble. The polymers were grafted onto the surface of polystyrene microplates. The poly-TBA polymers yielded a detection limit of 8 pM atrazine whereas for the poly-TBA-APBA plates it was lowered to 0.7 pM after 5 h of incubation. However, a 10-20% decrease in the polymer affinity was observed after 2 months. [Pg.147]

On-chip derivatization with 25 pM dichlorotriazine fluorescein (DTAF)... [Pg.862]

Triazole-substituted piperazine amides mimicking )8-tum stractures were reported by the Burgess group. Fifteen piperazine amides were prepared and isolated in high yields (23, 71-96%, Scheme 10.7) and used in couplings with dichlorotriazine derivatives (tagged with fluorescein or an alkyne) to afford combinatorial libraries 24, which were... [Pg.290]


See other pages where Fluorescein dichlorotriazine is mentioned: [Pg.213]    [Pg.1261]    [Pg.599]    [Pg.181]   
See also in sourсe #XX -- [ Pg.44 ]




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