Flow behavior of erythrocytes

addition of calcium ions (increase in erythrocyte rigidity) 

storing the sample for at least 4 h (cellular ageing, depletion of ADP). 

The following procedure can be used to evaluate the effect of test compounds on the flow behavior of erythrocytes. 

Membrane filter (Nuclepore Corp.) pore diameter 5-10 pm, pore density 4 x 105 pores/cm2. 

Blood is collected from Beagle dogs, weighing 12-20 kg, or from rabbits, weighing 1.2-2.5 kg, or from Wistar rats, weighing 150-300 g. The test subjects receive the test compound by oral, subcutaneous or intravenous administration 15, 60, 90 or 180 min before the withdrawal of blood. 

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The statistical significance is evaluated according to the procedure described for flow behavior of erythrocytes described above. 

The flow of leukocytes was studied in square capillaries fabricated on a Si chip, and sealed with a PDMS or Pyrex cover plate. This capillary size (cross section of 4 pm2) is similar to the diameter of a human blood capillary, but is less than both the average diameter of a leukocyte cell (10 pm) and its nucleus (6 pm). Figure 8.32 shows the difference in the flow behavior of two leukocytes (possibly neutrophils) [1175]. Deformation-induced release of ATP from erythrocytes in PDMS channels was studied. The released ATP was detected by chemiluminescence using the luciferin/luciferase system [169]. 

Fig. 9. Retention behavior of blood cells on poly(HEMA co[jV-methyl-/V-(4-vinylphenethyl) ethylenediamine (HAV) (pH 7.2, 23 °C flow rate, 0.4 ml/min flow time, 3,5 min). Open circles, lymphocytes closed squares, platelets open triangles, erythrocytes AVEMA, N-methyl- V-(4-vinyl-phenethyl)ethylenediamine [Ref. 115]...

Teitel P (1977) Basic principles of the Filterability test (FT) and analysis of erythrocyte flow behavior. Blood Cells 3 55-70... 

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