Fibroblast growth factor, stability

Chong MW, et al. Stabilizers against heat-induced aggregation of RPR 114849, an acidic fibroblast growth factor (aFGF). Int J Pharmaceut 1998 167 25-36. 

D. B. Volkin, A. M. Verticelli, K. E. Marfia, C. J. Burke, H. Mach, and C. R. Middaugh, Sucralfate and soluble sucrose octasulfate bind and stabilize acidic fibroblast growth factor, Biochim. Biophys. Acta, 1203 (1993) 18-26. 

One example of the probable regulatory function of PolyPs in animals is the ability of these polymers when added to culture media to enhance the proliferation of normal human fibroblast cells. PolyPs also enhanced the mitogenic activities of acidic fibroblast growth factor (FGF-1) and basic fibroblast growth factor (FGF-2). A physical interaction between PolyP and FGF-2 was observed, which stabilized this protein. Furthermore, PolyPs facilitated the FGF-2 binding to its cell surface receptors (Shiba et al, 2003). 

Volkin, D. B., and Middaugh, C. R. (1996). The characterization, stabilization, and formulation of acidic fibroblast growth factor. In Formulation, characterization, and stability of protein drugs (R. Pearlman and Y. J. Wang, eds.), pp. 181-217. Plenum, New York. 

K. and Igarishi, K. (1988) Stabilizing basic fibroblast growth factor using protein engineering. Biochem. Biophys. Res. Commun. 151 701-708. 

Thomas, K.A., Ortega, S., Soderman, D., Schaeffer, M.-T., DiSalvo, J., Giminez-Gallego, G., Linemeyer, D., Kelly, L. and Menke, J. (1991) Structural modifications of acidic fibroblast growth factor alter activity, stability, and heparin dependence. Ann. N. Y. Acad. Sci. 638 9-17. 

C. J., Linhardt, R.J. and Middaugh, C.R. (1993a) Physical stabilization of acidic fibroblast growth factor by polyanions. ArcJi. Biochem. Biophys. 300 30-41. 

Fukunaga, K., Hijikata, S., Ishimura, K., et al. (1994) Aluminium p-cyclodexMn sulphate as a stabilizer and sustained-release carrier for basic fibroblast growth factor. J. Pharm. Pharmacol. 46 168-171. 

Therapeutic proteins have also been stabilized in PLGA millicylinders by neutralizing the acidic microenvironment with insoluble basic additives in combination with additional excipients that improve the intrinsic stability of the encapsulated protein. Two examples are provided below using t-PA and basic fibroblast growth factor. 

Wang YJ, Shahrokh Z, Vemuri S, Eberlein G, Beylin I, Busch M. Characterization, stability, and formulations of basic fibroblast growth factor. In Pearlman R, Wang YJ, eds. Formulation, Characterization, and Stability of Protein Drugs. New York Plenum Press, 1996 141—180. 

Venkataraman, G., Sasisekharan, V, Herr, AB. et al. 1996. Rreferential self-association of basic fibroblast growth factor is stabilized by heparin during receptor dimerization and activation. Proceedings of the National Academy of Sciences 93 845-850. 

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