Fibrinogen crystal structure

Spraggon, G., et al. Crystal structures of fragment D from human fibrinogen and its crosslinked counterpart from fibrin. Nature 389 455-462, 1997. 

Crystal structure of modified bovine fibrinogen PDB ID IDEQ IFZA... 

View of a modified bovine fibrinogen molecule. The 45-nm-long disulfide-linked dimer is composed of three nonidentical polypeptide chains. The N termini of the six chains from the two halves come together in the center in a small globular "disulfide knot." The C termini form globular domains at the ends. The 340-kDa molecule has been treated with a lysine-specific protease which has removed portions of two chains to give the 285-kDa molecule whose crystal structure is shown. Arrows point to attached oligosaccharides. From Brown et at. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 85-90. Courtesy of Carolyn Cohen. 

Yang, Z., Mochalkin, I., Veerapandian, L., Riley, M., and Doolittle, R.F. (2000). Crystal structure of native chicken fibrinogen at 5.5 A resolution. Proc. Natl. Acad. Sci. 97, 3907-3912. 

Yang, Z., Kollman, J. M., Pandi, L., and Doolittle, R. F. (2001). Crystal structure of native chicken fibrinogen at 2.7 A resolution. Biochemistry 40, 12515-12523. Yonekura, K., Maki-Yonekura, S., and Namba, K. (2003). Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy. Nature 424, 643-650. Zhang, L., and Hermans, J. (1993). Calculation of the pitch of the a-helical coiled coil An addendum. Proteins 17, 217-218. 

The crystal structure of the central region of bovine fibrinogen, a fragment E, provided a view of the central region of the molecule and proximal portions of the coiled coil (Madrazo et al, 2001). The two halves of the molecule are joined together at the center in an extensive... 

The crystal structures of fragment D from several recombinant fibrinogens has demonstrated that these mutant molecules are excellent models for the study of structure-function relationships, since the polypeptide chains are folded properly with only local changes (Kostelansky et al., 2002). Moreover, these studies allow testing of hypotheses about the effects of a particular mutation on structure and internal interactions within the molecule, which then can be correlated with the effects on fibrin polymerization or platelet aggregation (Kostelansky et al., 2004a,b). 

Kostelansky, M. S., Betts, L., Gorkun, O. V., and Lord, S. T. (2002). 2.8 A crystal structures of recombinant fibrinogen fragment D with and without two peptide ligands GHRP binding to the b site disrupts its nearby calcium-binding site. Biochem. 41, 12124-12132. 

J.H. Brown, N. Volkmann, G. Jun, A.H. Henschen-Edman, and C. Cohen. 2000. The crystal structure of modified bovine fibrinogen Proc. Natl. Acad. Sci. U. S. A. 97 85-90. (PubMed) (Full Text in PMC)... 

Yang Z, MochaUdn I, Doolittle RF (2000) A model of fibrin formation based on crystal structures of fibrinogen and fibrin fragments complexed with synthetic peptides. Proc Natl Acad Sci USA 97 14156-14161... 

The crystal structure of approximately 70% of the residues of chicken fibrinogen has become available from the laboratory of Doolittle and coworkers as shown in Figure 7.27B. The (Aa)2(BP)2(y)2 subunit structure combines to form a flattened sigmoidal or S-like shape, which with the missing sequences will be indicated as (aPya PY)-The amino termini of all six chains, the two chains—aa, PP, and y/—all meet in the central part of the structure. 

G. Spraggon, S.J. Everse, and R.S. Doolittle, Crystal Structures of Fragment D from Human Fibrinogen and its Crosslinked Counterpart from Fibrin. Nature, 389,455-462,1997. 

Donahue, J. P., Patel, H., Anderson, W. F., and Hawiger, J. (1994). Three-dimensional structure of the platelet integrin recognition segment of the fibrinogen 7 chain obtained by carrier protein-driven crystallization. Proc. Natl. Acad. Sci. USA 91, 12178-12182. 

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