Fibre optics bioluminescence

Gautier S.M., Blum L.J., Coulet P.R., Multifunction fibre-optic sensor for the selected bioluminescent flow determination of ATP or NADH, Anal. Chim. Acta 1990 235 243-253. 

S. M. Gautier, I. J. Blum, andP. R. Coulet, Bioluminescence-based fibre optic sensor with entrapped co-reactant An approach for designing a self-contained biosensor, Anal. Chim. Acta 243, 149-156 (1991). 

Figure 3.6 — (A) Fibre-optic biosensor system a septum b needle guide c thermostated reaction vessel d fibre bundle e enzyme membrane f screw cap g stirring bar h reaction medium i black PVC jacket j 0-ring. (B) Continuous-flow fibre-optic sensor system for the bioluminescence determination of NADH. (Reproduced from [41] with permission of Marcel Dekker, Inc.)...

Gautier, S. M., Blum, L. J., and Coulet, P. R., Multi-function fibre-optic sensor for the bioluminescent flow determination of ATP or NADH. Ana/. Chim. Acta 235,243-253 (1990). Gautier, S. M., Blum, L. J., and Coulet, P. R., Bioluminescence-based fibre-optic sensor with entrapped co-reactant An approach for designing a self-contained biosensor. Ana/. Chim. Acta 243, 149-156 (1991). 

Blum LJ and Gautier SM (1991) Bioluminescence- and chemiluminescence-based fibre optic sensors. In Blum LJ and Goulet PR (eds.) Biosensor Principles and Applications, pp. 213-247. New York Dekker. 

Bioluminescence and chemiluminescence are very powerful analytical tools, since in addition to the direct measurement of ATP, NAD(P)H or hydrogen peroxide, any compound or enzyme involved in a reaction that generates or consumes these metabolites can be theoretically assayed by one of the appropriate light-emitting reactions. Some of these possibilities have been exploited for the development of optical fibre sensors, mainly with bacterial bioluminescence and with luminol chemiluminescence. 

Figure 7. (a) Flow diagram of the optical fibre continuous-flow system for bioluminescence and chemiluminescence measurements S, sample C, carrier stream PP, peristaltic pump IV, injection valve W, waste FO, optical fibre FC, flow-cell, (b) Details of the optical fibre biosensor/flow-cell interface a, optical fibre b, sensing layer c, light-tight flow-cell d, stirring bar. 

Since ideally, a biosensor should be reagentless, that is, should be able to specifically measure the concentration of an analyte without a supply of reactants, attempts to develop such bioluminescence-based optical fibre biosensors were made for the measurements of NADH28 30. For this purpose, the coreactants, FMN and decanal, were entrapped either separately or together in a polymeric matrix placed between the optical fibre surface and the bacterial oxidoreductase-luciferase membrane. In the best configuration, the period of autonomy was 1.5 h during which about twenty reliable assays could be performed. 

Table 2. Performances of batchwise and flow injection analysis (FIA) bioluminescence-based optical fibre sensors.

---