Fi-glucuronidase

Dissociation Constants (Kx) for Various ff-o-Glucosiduronic Acids Treated as Competing Substrates in the Hydrolysis of Phenolphthalein fi-o-Glucosiduronic Acid by Mammalian and Non-mammalian fi-Glucuronidase... 

FIGURE 10.14 Molecular-ion chromatograms of deprotonated DCL-AG, zw/z 470, in negative-ion ESI-MS mode. (A) One and a half (1.5) hours biosynthesis solution to generated primary AG and its isomers. (B) AG biosynthesis solution treated with fi-glucuronidase, showing enzyme hydrolysis-resistant rearranged AG isomers. 

Our studies on biologically active substances of this plant led to isolation of diterpenoids showing cytotoxicity [8], inhibitory effects on fi-glucuronidase [9], H+,K+-ATPase [10-12], replication of herpes simplex virus type 1 (HSV-1) [8,13,14] and bone resorption stimulated by parathyroid hormone (PTH) [15], and antitumor [16] and antitumor promoting effects [17]. In addition, some flavonoids possessing cytotoxicity [18], inhibitory activity against [3-glucuronidase [19] and antiviral activity [20] were also obtained from whole plants. 

Organisms Containing Endo-fi-glucuronidase Type Hyaluronidases... 

Figure L Possible monomer designs for the synthesis of HA and Ch. Allows show potential bonds for enzymatic cleavage by HAase (black arrows) and endo-fi-glucuronidase (white arrows).

Urine Hydrolyse 2 mL sample with 20 pL enzyme solution (fi-glucuronidase), 60 min, 60 °C Adjust sample pH to 8-9 with 0.1 N sodium carbonate solution... 

Sands, M. S. and Birkenmeier, E. FI. (1993). A single-base-pair deletion in the beta-glucuronidase gene accounts for the phenotype of murine mucopolysaccharidosis type VII. Proc. Natl. Acad. Sci. USA 90(14), 6567-6571. 

Reddy, B.S., Mangat, S., Weisburger, J.FI., and Wynder, E.L., Effect of high-risk diets for colon carcinogenesis on intestinal mucosal and bacterial beta-glucuronidase activity in F344 rats. Cancer Res., 37, 3533-3536,1977. 

Fig. 2. Histograms of liver glucuronidase distribution indices in DBA, C3H, Fi, and backcross animals. Individual livers were homogenized in 0.02 M imidazole buffer (pH 7.4) and centrifuged at 100,000 g for 30 minutes. The supernatant and sediment enzymes represent the lysosome and microsome activities, respectively. The distribution index is defined as the total lysosomal activity minus total microsome activity divided by the total liver activity (Paigen and Ganschow, 1965).

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