Ferrocene conjugated oligonucleotide

Figure 10.14 (a) Schematic representation of hybridization between ferrocene-conjugated oligonucleotide immobilized on gold electrode and its complementary strand, (b) Cyclic voltammograms recorded at v — 2(X) V/s and dependence of the anodic peak current on scan rate, normalized versus v1/2. Reprinted with permission from Ref. 76. Copyright 2003 American Chemical Society. 

The direct detection of DNA via conformational changes of ferrocene-conjugated DNA stem-loop (or hair-pin) structure onto electrode surface induced by the hybridization was also proposed (Fig. 11.1c) [4, 20]. The conformational change induces the displacement of ferrocene molecules from the electrode surface, resulting in a drop in peak redox current measured by cyclic voltametry. The detection limits were 115 fM for 24-base oligonucleotide [20] and 10 pM for 17-base oligonucleotide [4], respectively. Furthermore, the difference between... 

M. Nakayama, T. Ihara, K. Nakano, and M. Maeda, DNA sensors using a ferrocene-oligonucleotide conjugate. Talanta 56, 857-866 (2002). 

Ihara, T. Maruo, Y. Takenaka, S. Takagi, M. Ferrocene-oligonucleotide conjugates for electrochemical probing of DNA. Nucl. Acid. Res. 1996, 24, 4273-1280. 

Nakayama, M. Ihara, T. Nakano, K. Maeda, M. DNA sensors using a ferrocene-oligonucleotide conjugate. Talanta 2002, 56, 857-866. Zauner, G. Wang, Y. T. Lavesa-Curto, M. MacDonald, A. Mayes, A. G. Bowater, R. P. Butt, J. N. Tethered DNA hairpins facilitate... 

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