Features of the basic instrument

FI0irel Schematic diagram of a conventional stopped-flow spectrophotometer. 

There are now some excellent modem instmments available that are sensitive, stable and equipped with good, user-ftiendly, software for example  

The examples we have chosen to illustrate have been obtained using the Applied Photophysics instrument SX.18MV, which is provided with a PMT detection system and supplemented with a diode array for collecting spectra during a reaction time course. 

1 Fill working syringes with water or buffer. 

2 Select a wavelength where the lamp output is high and the PMT is most sensitive, usually somewhere between 500-600 nm. 

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