Fd bacteriophage

Fig. 12. (a) Experimental PISEMA spectrum of uniformly " N-labeled Y21M fd bacteriophage... 

Newman et al. (1974) have recently studied the concentration-dependence of the diffusion coefficient and the sedimentation coefficient in a highly monodisperse solution of the single-stranded circular DNA from the fd Bacteriophage. Their results are shown in Fig. 13.5.3. From these data it is possible to determine the coefficients in the expression... 

Rabbit anti-fd Bacteriophage (Sigma, cat. no. B7786) Peroxydase-coupled mouse anti-rabbit IgG (Sigma, cat. no. A1949) Rabbit anti-Evasin (or other specific antibody targeting the protein of interest)... 

Add 100 pL/well of the primary antibody, a rabbit anti-fd Bacteriophage diluted 1 1000 in PBS containing 1 % milk. Incubate for 1 h with gentle agitation (450 rpm). 

NMR studies of the motions of the aromatic amino acids of the fd bacteriophage protein were made, and the results are shown in Fig. 8.12. The experimental spectrum fits the calculated flip-averaged spectrum better than it fits the rotationally averaged spectrum. Therefore the rings are executing 180 flips in less than 1 ns, and probably in less than 0.01 ns. Additionally, the experimental spectrum is narrower than expected from the static chemical-shift tensor, and so additional molecular motion of the main chain is also involved. 

Figure 5.15 The filamentous single-stranded DNA bacteriophage fd. Orientation of the proteins and genes in the virion. Note the intergenic space which contains the origin of DNA synthesis. Gene cloning is done in this intergenic space.

Fig. 5.1 Schematic drawing of membrane association modes of peptides A Integral membrane proteins (1) major fd coat protein gpVIII of bacteriophage Ml 3 (pdb lfdm), anchored by an 18-residue trans-membrane hydrophobic helix (2) bovine rhodopsin, a 7 trans-membrane domain (G-protein-coupled) receptor (pdb lf88) (3) ion channel peptaibol Chrysospermin C (pdb lee7), and B Peripheral membrane proteins (1) neuro-...

Filamentous bacteriophages. Bacteriophages of the Ff family include the fd, fl, and M13 strains.313,32-36 Phage Ml 3 is widely used in cloning genes and for many other purposes (Chapter 26). The genome is a circular, single-stranded DNA of 6400 nucleotides... 

Fig. 2. The structure of M13 (fd), according to Makowski [10,35], and X bacteriophage, showing the proteins which have been tested or implemented in phage display. The phage are both oriented so that their feet , which they use for attachment to, and penetration through, the cell membrane, are at the top of the diagram. The M13 proteins are numbered with roman numerals. X (lambda) proteins are designated alphabetically, i.e. pV is protein Vee not five, and pD is protein Dee not fifty.

The E. coli filamentous bacteriophages, such as M13, fd, and fl, are the most commonly used viruses in biological display systems. Their genomes are small... 

Holliger P, Riechmann L, A conserved infection pathway for filamentous bacteriophages is suggested by the structure of the membrane penetration domain of the minor coat protein g3p from phage fd, Structure, 5 265-275, 1997. 

Lambda phage is closely related to other bacteriophages, and these are called T3, T7, T5, Mu, T2, and T4. All of these phages corcsist of a double-stranded DNA genome, an icosahedral head, a long cylindrical tail, and a variable number of tail filaments. The related filamentous phages are Ml3, fl, and fd. 

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