Fatty acid ADAM esters

Schmidt A, Vogel RL, Witherup KM. Rutledge SJ, Pitzenberger SM, Adam M, et al. Identification of fatty acid methyl ester as naturally occurring transcriptional regulators of the members of the peroxisome proUferator-activated receptor family. Lipids 1996 31 1115-1124. 

Nimura and Kinoshita [96] developed a derivatization technique for the analysis of fatty acids that is based on 9-anthryldiazomethane (ADAM). The latter is very stable in solution and reacts with fatty acids at room temperature without a catalyst to yield strongly fluorescent esters, which can be chromatographed on an ODS phase with acetonitrile/water mixtures. The derivatization of fatty acid is carried out with a 0.1% methanohc solution of 9-anthryldiazomethane, which is prepared via oxidation of 9-anthraldehyde-hydrazone following a procedure by Nakaya et al. [97]. The fluorescence and excitation spectra of fatty acid-methyl-anthracene esters exhibit maxima at 412 and 365 nm, respectively. As an example, Figure 8.62 shows the separation of ADAM derivatives, a mixture of saturated and unsaturated long-chain fatty acids, which can be detected down to the lowest picomole range. 

Contado MJ, Adams J. Collision-induced dissociations and B/E linked scans for structural determination of modified fatty acid esters. Anal Chim Acta. 1991 246 187-97. 

The purpose of this review is to compare the various approaches used to improve cellular lipid preservation and discuss the results obtained in the study of different lipid compounds in several tissues. This review will be confined mostly to metabolic studies performed in mammals in vivo or in vitro and will encompass the following lipid classes triglycerides, free fatty acids, cholesterol (free and ester), and phospholipids. No attempt will be made to review histo-chemical approaches to localization of lipids or radioautographic localization of steroid hormones, as these subjects were dealt with recently in a detailed way by Adams (1969) and by Stumpf (1970). 

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