Expressing organophosphorus hydrolase

Comparison of whole cell biocatalytic reaction kinetics for recombinant Escherichia coli with periplasmic-secreting or cytoplasmic-expressing organophosphorus hydrolase... 

A. Mulchandani, I. Kaneva, and W. Chen, Biosensor for direct determination of organophosphate nerve agents using recombinant Escherichia coli with surface-expressed organophosphorus hydrolase. 2. Fiber-optic microbial biosensor. Anal. Chem. 70, 5042-5046 (1998). 

Richins RD, Kaneva I, Mulchandani A et al. Biodegradation of organophosphorus pesticides by surface-expressed organophosphorus hydrolase. Nat Biotechnol 1997 15(10) 984-987. 

Mulchandani P, Chen W, Mulchandani A et al. Amperometric microbial biosensor for direct determination of organophosphate pesticides using recombinant microorganism with surface expressed organophosphorus hydrolase. Biosens Bioelectron 2001 16(7-8) 433-437. 

Mulchandani A., Kaneva I., and Chen W., Detoxification of organophosphate nerve agents by immobilized Escherichia coli with surface-expressed organophosphorus hydrolase, Biotechnol. Bioeng., 63, 216-223, 1999. 

Richins, R.D., A. Mulchandani, and W. Chen. 2000. Expression, immobilization, and enzymatic characterization of cellulose-binding domain-organophosphorus hydrolase fusion enzymes. Biotechnology and Bioengineering 69(6) 591-596. 

Wu C.F., Cha H.J., Rao G., Valdes J.J. and Bentley W.E. 2000. A green fluorescent protein fusion strategy for monitoring the expression, cellular location, and separation of biologically active organophosphorus hydrolase. Appl. Microbiol. Biotechnol., 54, 78-83. 

Wang, X., Wu, N., Guo, J., et al., 2008. Phytodegradation of organophosphorus compounds by transgenic plants expressing a bacterial organophosphorus hydrolase. Biochem. Biophys. Res. Commun. 365, 453-458. 

Millard, C.B., Lockridge, O., Broomfield, C.A. (1995). Design and expression of organophosphorus acid anhydride hydrolase activity in human butyrylcholinesterase. Biochemistry 34 15925-33. 

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