Experimental procedure for preparation of activated donors

Notes and discussion. This pragmatic method allows preparation of pNP glycosides on a gram scale. In our hands, the material obtained after freeze drying is typically analytically pure and in these cases the final recrystallization may be omitted. 

L-Rhamnopyranose monohydrate (11.10 g, 60.9 mmol) Sodium acetate (5.5 g, 67.0 mmol) 

Heater stirrer with thermostat thermometer Oil bath 

Rotary evaporator Water aspirator 1000 ml round bottom flask Freeze dryer 

Special precautions. Care should be taken with the extraction of the pNP-glycoside melt with chloroform a temperature of 50 °C proves ideal for keeping the melt hquid but avoids overly rapid evaporation of the chloroform being used for the extraction. 

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