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Pooling, experimental design

Methodological controls. Some published studies on the effectiveness of hypericum have experimental design flaws, but there are several that are methodologically controlled, employing double-blind, randomization, and placebo controls. Randomized, placebo-controlled studies were summarized and evaluated in a meta-analysis by Linde and colleagues (1996). The combined subject pool was 1,757 outpatients with mild to... [Pg.269]

The experimental design is rather straightforward. The mRNAs from all fest cell lines were converted to Cy5-labeled cDNAs by RT. They were compared against a reference of pooled mRNAs from 12 separate cell lines that had been reverse transcribed to produce a Cy3-labeled cDNA reference. This pool represented the maximum diversity across the 60 cell lines and was used as the reference throughout the study. [Pg.164]

For our validations, a CVp is a pooled estimate calculated from the particular type of statistical data set (36 samples) described earlier in the Statistical Experimental Design section of this report. A statistical procedure is given in Hald JL for determining an upper confidence limit for the coefficient of variation. This general theory had o be adapted appropriately for application to a pooled CVp estimate. For this design, and under the stated assumptions, there is a one-to-one correspondence between values of CVp and upper confidence limits for CVp. Therefore, the confidence limit criterion given above is equivalent to another criterion based on the relationship of CVp and its critical value. The... [Pg.508]

Chrysikopoulos CV, Lee KY, Harmon TC (2000) Dissolution of a well-defined trichloroethylene pool in saturated porous media experimental design and aquifer characterization. Water Resour Res 36 1687-1696... [Pg.132]

Alban, A., David, S. O., Bjorkesten, L, Andeesson, C., Sloge, E., Lewis, S., Currie, I. (2003). A novel experimental design for comparative two-dimensional gel analysis two-dimensional difference gel electrophoresis incorporating a pooled internal standard. Proteomics 3, 36-44. [Pg.53]

In particular, for small experimental designs, in which only few arrays are available for each biological condition, pooling could actually improve accuracy... [Pg.1088]

D.G. (2002) Identification of the sources of error in allele frequency estimations from pooled DNA indicates an optimal experimental design. Ann. Hum. Genet., 66 (5-6). 393 05. [Pg.237]

One of the most commonly used baths is a variant of the original bath developed by Mcllwain and his co-workers, first described by Schwartz-kroin (1975) (Fig. 2). In this bath, the slice sits in the media/gas interface, over a pool containing approximately 2 ml of solution, which is perfused from below, at a rate of less than 2 ml/min. As mentioned elsewhere, the slice appears to extract oxygen from the gaseous phase and, provided a flow of less than 2 ml/min is demanded by the experimental design, stable intracellular records can be maintained, at least from the larger cells of the slice, for periods in excess of 4 hr. [Pg.103]


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See also in sourсe #XX -- [ Pg.209 ]




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