Experimental Aspects of Intracellular SERS Studies

In the intracellular studies lateral resolution and detection sensilivily are two major parameters influencing the vibration information obtained. The lateral resolution is 

Viruses can be identified and classified by using SERS spectroscopy after adsorption on Ag NPs (Bao et al. 2001) or solid metal surfaces (Alexander 2008). Obtained SERS spectra contain spectral information about capsid (protein shell) of the vims due to Raman bands of amino acids containing COOH-, NH2- and 

SH- groups (Bao et al. 2001). Insect nuclear polyhedrosis virus (Bao et al. 2001), parapoxviruses (Alexander 2008) and respiratory syncytial virus (Shanmukh et al. 2008) were studied. Higher sensitivity and selectivity were obtained by a combination of AFM and SERS in a study of nm-sized feline caliciviruses (Porter et al. 2006) and by TERS in the case of single tobacco mosaic vims at a molecular level (Cialla et al. 2009). 

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