Excitation contour

Measure cell fluorescence by LSC using 488 nm argon ion laser for excitation. Contour on red fluorescence signal and measure intensity (integrated values) of green fluorescence of FITC-anti BrdU MAb at 530 20 nm and red fluorescence of PI at >600 nm. 

Figure Al.6.27. Equipotential contour plots of (a) the excited- and (b), (c) ground-state potential energy surfaces. (Here a hamionic excited state is used because that is the way the first calculations were perfomied.) (a) The classical trajectory that originates from rest on the ground-state surface makes a vertical transition to the excited state, and subsequently undergoes Lissajous motion, which is shown superimposed, (b) Assuming a vertical transition down at time (position and momentum conserved) the trajectory continues to evolve on the ground-state surface and exits from chaimel 1. (c) If the transition down is at time 2 the classical trajectory exits from chaimel 2 (reprinted from [52]).

Figure A3.13.6. Time evolution of the probability density of the CH cliromophore in CHF after 50 fs of irradiation with an excitation wave number = 2832.42 at an intensity 7q = 30 TW cm. The contour...

Figure 4. Relaxed triangular plot [68] of the Li3 first-excited state potential energy surface using hyperspherical coordinates. Contours are given by the expression E (eV) =-0.56-1-0.045(n — 1) with n = 2,3,. The dissociation limit indicated by the dense contouring implies...

Room-temperature fluorescence (RTF) has been used to determine the emission characteristics of a wide variety of materials relative to the wavelengths of selected Fraunhofer lines in support of the Fraunhofer luminescence detector remote-sensing instrument. RTF techniques are now used in the compilation of excitation-emission-matrix (EEM) fluorescence "signatures" of materials. The spectral data are collected with a Perkin-Elraer MPF-44B Fluorescence Spectrometer interfaced to an Apple 11+ personal computer. EEM fluorescence data can be displayed as 3-D perspective plots, contour plots, or "color-contour" images. The integrated intensity for selected Fraunhofer lines can also be directly extracted from the EEM data rather than being collected with a separate procedure. Fluorescence, chemical, and mineralogical data will be statistically analyzed to determine the probable physical and/or chemical causes of the fluorescence. 

Figure 19. Outgoing waves on the first three electronic states of H2O following excitation of the (B Ai) (X Ai) transition. Green and red lobes have positive and negative amplitudes, respectively. The gray-fiUed contour on the B surface represents the Franck-Condon region of excitation from the ground state. Reprinted from [75] with permission from the American Association for the Advancement of Science. (See color insert.)...

Figure 12. Potential energy contour plots for He + I Cl(B,v = 3) and the corresponding probability densities for the n = 0, 2, and 4 intermolecular vibrational levels, (a), (c), and (e) plotted as a function of intermolecular angle, 0 and distance, R. Modified with permission from Ref. 40. The I Cl(B,v = 2/) rotational product state distributions measured following excitation to n = 0, 2, and 4 within the He + I Cl(B,v = 3) potential are plotted as black squares in (b), (d), and (f), respectively. The populations are normalized so that their sum is unity. The l Cl(B,v = 2/) rotational product state distributions calculated by Gray and Wozny [101] for the vibrational predissociation of He I Cl(B,v = 3,n = 0,/ = 0) complexes are shown as open circles in panel (b). Modified with permission from Ref. [51].

Exciplexes are complexes of the excited fluorophore molecule (which can be electron donor or acceptor) with the solvent molecule. Like many bimolecular processes, the formation of excimers and exciplexes are diffusion controlled processes. The fluorescence of these complexes is detected at relatively high concentrations of excited species, so a sufficient number of contacts should occur during the excited state lifetime and, hence, the characteristics of the dual emission depend strongly on the temperature and viscosity of solvents. A well-known example of exciplex is an excited state complex of anthracene and /V,/V-diethylaniline resulting from the transfer of an electron from an amine molecule to an excited anthracene. Molecules of anthracene in toluene fluoresce at 400 nm with contour having vibronic structure. An addition to the same solution of diethylaniline reveals quenching of anthracene accompanied by appearance of a broad, structureless fluorescence band of the exciplex near 500 nm (Fig. 2 )... 

Figure 9. Comparison of ab initio (full line) and ab m/rfo/interpolated (dashed line) potential energy surfaces for the first electronically excited state of Li + H2 system restricted to C2v geometry. Contours are labeled in eV. (Figure adapted from Ref. 125.)...

Van Duuren [37] examined the use of emission and excitation spectra in the identification of aromatic hyorocarbons. Contour diagrams of fluorescence activity at various excitation and emission wavelengths have been used as a means of identifying petroleum residues. 

Figure 3.77 Leading donor-acceptor interactions in a 3n 7t excited aminodi-alkyl ketone, showing filled (ctqn1) and empty (

Figure 3.77 depicts contour diagrams of the leading excited-state TB interactions... 

Figure 1. Contour plots of the Husimi distributions of the ground (b), first excited... 

Fig.4 (see p. 74/75) shows all localized orbitals for the ground state of the BH molecule and the 12 excited state of B2.37) These are again rotationally symmetric orbitals, i.e., sigma type orbitals, and the complete contour surfaces can be obtained by spinning around the indicated axis. In all orbitals shown the outermost contour corresponds to a wavefunction value of 0.025 Bohr-3/2. For all valence shell orbitals the increment from one contour to another is 0.025 Bohr-3/2. For the inner shells the increment is again 0.2 Bohr 3/2, but only three contours and the wavefunction values at the nuclear positions are shown. 

LIF excitation spectra were recorded for alkyl aminobenzoates (165) under free jet conditions. The partially resolved band contours were different for the various compounds... 

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