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Exchange Chromatography IEC

In this technique, separation is based on the exchange of ions (anions or cations) between the mobile phase and the ionic sites on a stationary phase bound to a support material in the column. A charged species is covalently bound to the surface of the stationary phase. The mobile phase, typically a buffer solution, contains a large number of ions that have a charge opposite to that of the surface-bound ions. These mobile-phase ions, referred to as counterions, establish an equilibrium with the sta- [Pg.191]

Practical Guide to ICP-MS A Tutorial for Beginners, Second Edition [Pg.192]

FIGURE 18.3 Principle of separation using anion exchange chromatography. [Pg.192]

In ion-exchange chromatography (IEC), the mobile phase modulator is typically a salt in aqueous solution, and the stationary phase is an ion-exchanger. For dilute conditions, the solute retention factor is commonly found to be a power-law function of the salt normality [cf. Eq. (16-27) for ion-exchange equilibrium]. [Pg.45]

Amino acids Adsorption on ferric oxide Io exchange chromatography (IEC) 3 ig/l [511,512]... [Pg.438]

Hydrophobic interaction chromatography (HIC) occupies a unique niche in the field of analytical chromatography. A particular advantage of HIC is its unique selectivity. Whereas ion-exchange chromatography (IEC) principally reveals differences based on the surface charge of native proteins, HIC reveals differences based principally on their surface hydrophobicity. HIC is complementary to reversed-phase chromatography (RPC) in a different sense. Whereas HIC discriminates primarily on the basis of surface hydrophobicity, RPC principally reveals differences based on total hydrophobicity of all the hydrophobic residues of denatured proteins. [Pg.81]

Ion-exchange chromatography (IEC), on the basis of differences in the pKa values of the functional groups of the side chains resulting in changes in the ionization function of pH... [Pg.133]

In ion-exchange chromatography (IEC), species are separated on the basis of differences in electric charge. The primary mechanism of retention is the electrostatic attraction of ionic solutes in solution to fixed ions of... [Pg.38]

Ion Exchange Chromatography. In ion exchange chromatography (IEC), the solid stationary phase is comprised of ionic material. This serves as an ion exchanger and separation is achieved primarily on the basis of the relative affinities of... [Pg.85]

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IEC

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