Examples of Protein Engineering

Excellent examples of protein engineering work on dioxygenases are given in Section 10.8, changing the regio- and stereoselectivity on Rieske non-heme iron... 

There are many examples of protein engineering that use rational design and directed evolution. 

An early example of protein engineering of an OYE pertains to the reductase from S. pastorianus as a catalyst in the stereoselective reduction of hulky 3-aIkyl-suhstituted 2-cyclohexenone derivatives in the presence of an NADPH regeneration system [35]. 

TABLE 17.1 Examples of Protein Engineering Approaches Used to Improve or Modify Aromatic Hydrocarbon Dioxygenases... 

Successful applications of protein engineering include the following examples ... 

In Nature, there are many examples of protein and peptide molecular self-assembly. Of the genetically engineered fibrous proteins, collagen, spider silks, and elastin have received attention due to their mechanical and biological properties which can be used for biomaterials and tissue engineering. 

Capacity to introduce natural bioactive peptide sequences (protein mimicry) With an adequate understanding of protein engineering, there exists an essentially unlimited capacity to mimic chosen elements of more complex proteins in terms of both structure and function. Specific biologically active sequences can be introduced with ease. Examples are sites at which selective enzymes can catalyze a desired reaction, sequences for selective cell attachment, selective attachment to diseased cells for drug delivery, and so forth. 

One of the most pretentious approaches for future biochemical engineering would consist of tailoring proteins to desired fvmctions by protein engineering. Pioneering work has for example been done in the area of biocatalysis, but it is commonplace that rational exploitation of protein engineering will require an enormous amount of additional knowledge on the primary - tertiary structure - function relationships. These again emphasize the importance of thermodynamics in the area of protein stability. 

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