Evidence for Translocation of Enzymes during Smooth Muscle Activation

EVIDENCE FOR TRANSLOCATION OF ENZYMES DURING SMOOTH MUSCLE ACTIVATION 

Cell fractionation studies in bovine carotid artery first demonstrated that phorbol esters, histamine, angiotensin, and endothelin, but not KCl, induced a translocation of PKC from the cytosol to the membrane fraction (Haller et al., 1990), although the identity of the membranes involved was not determined (Table I). Work with the non-isoform-specific fluorescent PKC probe Bodipy phorbol (Khalil and Morgan, 

Enzyme Cell type Resting state Activated state Reference 

Protein kinase C Bovine carotid artery Cytosolic fraction Membrane fraction Haller et al. (1990) 

3-PKC Carotid artery Cytosolic fraction Particulate fraction Singer et al. (1992) 

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