Etodolac sensitivity

A simple, sensitive, and reproducible fluorimetric method for the determination of etodolac in bulk powder or dosage forms has been reported [19]. The method involves measurement of the native fluorescence at a wavelength of 345 nm, when ethanolic solutions of the drug were excited at 235 nm. The calibration was found to be linear over the concentration range of 96 to 640 ng/mL. 

A sensitive reverse-phase HPLC method has been developed for the analysis of etodolac in tablet formulation [22]. The chromatographic separation was achieved using a reverse-phase Cu column, having dimensions of 3.3 cm x 0.46 cm i.d. (3 pm particles) and which was maintained at 30°C. The mobile phase consisted of pH 6.0 phosphate buffer / methanol (60 40 v/v), and was eluted at 1 mL/min. Analyte detection was effected on the basis of UV detection at 230 nm. Diazepam was used as an internal standard. The sample preparation entailed grinding the etodolac tablets, followed by extraction with methanol (using sonication). A retention time of 1.46 min was obtained for etodolac under these conditions, and the method was found to be linear, precise, and accurate over the concentration range of 0.01 to 0.1 mg/mL. 

Cosyns, L. Spain, M. Kraml, M. Sensitive high-performance liquid chromatographic method for the determination of etodolac in serum. J.Pharm.ScL, 1983, 72, 275-277... 

Christie DJ, Mullen PC, Aster RH (1985) Fab-mediated binding of drug-dependent antibodies to platelets in quinidine- and quinine-induced thrombocytopenia. J Clin Invest 75 310-314 Cunha PD, Lord RS, Johnson ST, Wilker PR, Aster RH, Bougie DW (2000) Immune hemolytic anemia caused by sensitivity to a metabolite of etodolac, a nonsteroidal anti-inflammatory drag. Transfusion 40 663-668... 

We describe three simple, sensitive and reproducible spectrophotometric assays (A-C) for the determination of etodolac in pure form and in pharmaceutical formulations. Methods A and B are based on the oxidation of... 

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