Ether-soluble metabolites isolated

Figure 6. Ether-soluble metabolites isolated from the roots of peanut plants treated with FCNB. This represents 13.7% of the C isolated from the roots.

C]PCNB metabolism was studied in vivo with 30-day-old peanut plants grown in nutrient solution that contained 17.6 ppm [I CjPCNB. Plant tissue was extracted with cold 80t methanol 48 hr after final exposure to PCNB. The extracts were made aqueous and partitioned against chloroform at pH 5.5 and against ethyl ether at pH 2. Water-soluble, chloroform-soluble, and ether-soluble metabolites were isolated by various chromatographic methods and identified by mass spectrometry and/or by synthesis. The details of these studies have been published previously (, X ... 

C15H20O2, Mr 232.32, colorless crystals, mp. 82 °C, bp. 275 °C, [a] 3 +197° (CHCI3), almost insoluble in water, soluble in alcohol and ether. The name of this tricyclic sesquiterpene lactone is derived from the Asteraceae ]nula helenium originally indigenous to Central Asia. H. was isolated for the first time from the essential oil of the roots (Inula oil). H. is also an older synonym for helenalin as well as the name for a metabolite from Penicillium funiculosum (ribonucleoprotein). Because of its a-methylene-y-lactone structure H. is a contact allergen. 

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