Escherichia coli spheroplasts

Henner.W.D., I. Kleber, and R. Benzinger. 1973. Transfection of Escherichia coli spheroplasts. 3. Facilitation of transfection and stabilization of spheroplasts by different basic polymers./. Virol. 12 741—747. 

Benzinger, R., Kleber, I., Huskey, R. Transfection of Escherichia coli spheroplasts. 

The effects of ampicillin-induced spheroplast formation on the production of molecular hydrogen by Escherichia coli carrying out fermentation in a lactose-peptone broth with an osmolality of 342 mosmol/1 was investigated previously... 

M3. Malamy, M. H., and Horecker, B. L., Release of alkaline phosphatase from cells of Escherichia coli upon lysozyme spheroplast formation. Biochemistry 3, 1889-1893 (1964). 

Novobiocin also causes chaining in Streptococcus faecium [23], although this effect is not produced in all cocci [13]. The induction of spheroplasts in Escherichia coli by novobiocin has also been reported [24] and, in fact, the antibiotic has been recommended for preparing spheroplasts in Gramnegative bacteria [25]. In contrast, however, it has been shown by various workers [26-28] that novobiocin does not induce spheroplasts in Serratia marcescens or in various strains of E. coli and [28] that it may even prevent spheroplast induction caused by benzylpenicillin in hypertonic medium (Figure 2.1). 

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