Eschericha coli

Cloning and Expression of a Gene Coding for a Secondary Alcohol Dehydrogenase from Candida parapsilosis IFO 1396 in Eschericha coli 

The reductive reaction was carried out as described in Materials and Methods for 4-hydroxy-2-buta-none reductase, except that substrates and cofactors indicated in the table were used. Activity for 4-hydroxy-2-butanone was taken as 100%. 

Name CpSADH CPCR RECR Long-chain ADH 

The reaction conditions for the synthesis of (R)-l,3-BDO were optimized using recombinant E. coli cells [19]. The optimal pH of a reaction mixture was found to be pH 6.8. This result suggested that the rate-limiting step of enantioselective oxidation was a regeneration step of the coenzyme, NAD+, and that the optimal pH and/or the stablest pH for the regeneration of the coenzyme was around pH 6.8, since the optimal pH of CpSADH for the oxidation of (S)-l,3-BDO was 9.0 and CpSADH was stable within the range of 8.0-11.0. 

The effects of the concentrations of 1,3-BDO in the reaction mixture were studied. At the reaction containing 7% 1,3-BDO, the optical purity of (R)-1,3-BDO reached 97.6% ee after incubation of 17 hours, as shown in Fig. 7. 

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Cristobal, S., de Gier,J.-W., Nielsen, H., and von Heijne, G. (1999). Competition between sec- and tat-dependent protein translocation in Eschericha coli. EMBOJ. 18, 2982-2990. 

Prinz, W., Boyd, D., Ehrmann, M., and Beckwith, J. (1998). The protein translocation apparatus contributes to determining the topology of an integral membrane protein in Eschericha coli. J. Biol. Chem. 273, 8419-8424. 

Qi, H.-Y., and Bernstein, H. (1999). SecAis required for the insertion of inner membrane proteins targeted by the Eschericha coli signal recognition particle. J. Biol. Chem. 274, 8993-8997. 

Sjostrom, M., Wold, S., Wieslander, A., and Rilfors, L. (1987). Signal peptide amio acid sequences in Eschericha coli contain information related to final protein localization. A multivariate data analysis. EMBO J. 6, 823—831. 

Tsuchiya, H. M., Drake, J. F Jost, J. L. and Fredrickson, A. G. J. Bacteriol. 110 (1972) 1147. Predator-prey interactions of Dictyoselium discoidem and Eschericha coli in continuous culture. 

Ishizaki, K., Sawadaishi, K., Miura, K. and Shinriki, N. (1987) Effect of ozone on plasmid DNA of Eschericha coli in situ. Water Res. 21, 823-827. 

Cytochrome exists in a soluble form in erythrocytes and in a membrane bound form in microsomes. A soluble derivative of hepatic cyt bs with 93 amino acids can be isolated by treatment of microsomes with pancreatic lipase and this form has essentially the same amino acid sequence as the erythrocyte protein. Further treatment of the soluble form with trypsin cleaves two residues from the N-terminal and seven from the carboxylate-terminal and leaves the heme core with only 84 residues. Rat liver cyt b has been prepared by expression in Eschericha coli (E.coli). The structure of... 

An extract of Eschericha coli, free from hexoseisomerase, is capable of converting D-glucosamine 6-phosphate to D-fructose 6-phosphate plus ammonia. No added cofactors are required for this reaction. ... 

Eschericha coli, strain DH5a (Life Technologies). 

Electrocompetent JM109 Eschericha coli cells (Life Technologies, Merelbeke, Belgium). 

In trial 1 and 2, all participants used their own method and a common method. After trial 1 and 2, the most representative strains for coliforms Eschericha coli) and streptococci (five strains) were selected. As some methods already failed on the artificially contaminated samples of the first two trials, they were not used in trial 3 and 4. After the third trial, three groups of methods for each parameter appeared to be the most reliable. The participants selected these methods as reference methods for the next steps. Each participant applied the three methods on the set of real seawater samples together with the own method. The real seawater samples were transported overnight by plane to the central laboratory. Over the various trials, several participants identified bad practice or weak points in their own method(s) and were allowed to correct for them. Finally, three methods emerged as the most robust, reliable (comparable) and precise for each of the two (groups oO parameters. In total several thousands of determinations on samples of increasing difficulty were performed. The overall conclusions and the results have been published by the Commission [62]. 

Eschenlauer AC, StoupSK, SriencF, Somers DA. 1996. Production of HeteropolymeiicPolyhydroxyalkanoate in Eschericha Coli from a single carbon source. Int J Biol Macmol 19 121-130. 

Biol, prop. Activity against Staphylococcus aureus and Eschericha coli (702). 

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